Journal: Nature Communications

Article Title: Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin

doi: 10.1038/s41467-020-19425-1

Figure Lengend Snippet: N-cadherin was locally knockout around wounds on Ncad fl/lfl mice by injection of Cre-expressing AAV6-Cre-GFP virus. AAV6-GFP virus served as control. a Immunolabelling of N-cadherin on transverse cross-sections of harvested scars on 14-dpi. GFP indicates transduced cells. Dash lines outline the scar edges. b N-cadherin expression in GFP + cells based on immunofluorescence analysis. Data are normalised on the mean of N-cadherin expression in AAV6-GFP wounds. Mean ± SD, n = 5, p = 0.0003, unpaired two-tailed t -test. c Stereomicroscopic images of AAV6-Cre-GFP and AAV6-GFP treated scars at 14-dpi. The yellow dash lines indicate the scar edge. d Quantification of scar area based on histomorphometric analysis. Mean ± SD, n = 8, p = 0.0002, unpaired two-tailed t -test. e Masson’s trichrome stained vertical (upper panel) and transverse (lower panel) sections from AAV6-Cre-GFP and AAV6-GFP treated scars. The dash lines indicate scar width. f Quantification of scar width based on histomorphometric analysis. Mean ± SD, n = 5, p = 0.001, unpaired two-tailed t -test. g Fractal dimension and lacunarity analysis of AAV6-Cre-GFP and AAV6-GFP treated scars and adjacent normal skin. Mean ± SD, one-way ANOVA Tukey’s test, n = 8, p values from multiple comparisons are shown in the graph. Scale bars: a , d = 200 µm; b = 500 µm.

Article Snippet: Cre-expressing AAV6 (AAV6-Cre-GFP) was purchased from Addgene (Addgene #68544 ).

Techniques: Knock-Out, Injection, Expressing, Virus, Control, Immunofluorescence, Two Tailed Test, Staining